Proteomic Analysis of the Fusarium graminearum Secretory Proteins in Wheat Apoplast Reveals a Cell-Death-Inducing M43 Peptidase

Pengfeng Li; Ruihua Zhao; Ying Fang; Yujin Fan; Qianyong Hu; Wei Huang; Wujun Ma; Cuijun Zhang

doi:10.3390/jof11040240

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Proteomic Analysis of the Fusarium graminearum Secretory Proteins in Wheat Apoplast Reveals a Cell-Death-Inducing M43 Peptidase

Journal article

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Proteomic Analysis of the Fusarium graminearum Secretory Proteins in Wheat Apoplast Reveals a Cell-Death-Inducing M43 Peptidase

Pengfeng Li, Ruihua Zhao, Ying Fang, Yujin Fan, Qianyong Hu, Wei Huang, Wujun Ma and Cuijun Zhang

Journal of fungi (Basel), Vol.11(4), 240

2025

DOI: https://doi.org/10.3390/jof11040240

PMID: 40278061

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Abstract

<italic>Fusarium graminearum</italic>

wheat

secretory proteins

M43 peptidase

Fusarium graminearum, a highly destructive fungal pathogen, poses a major threat to wheat production. The apoplast is an important space for plant–pathogen interactions. However, no studies have been reported on the secretory proteins of F. graminearum in the wheat apoplast. In this study, we performed mass spectrometry analysis of F. graminearum secretory proteins in wheat apoplast and identified 79 potential secretory proteins. We identified a metalloprotease (referred to as Fg28) and demonstrated its capacity to induce cell death and reactive oxygen species (ROS) accumulation in Nicotiana benthamiana. Fg28 is strongly up-regulated in the early stages of infection and is secreted into the intercellular space of wheat cells. Full-length Fg28 is required to induce cell death in N. benthamiana. In addition, Fg28 induces an immune response that is independent of BAK1/SOBIR1 and EDS1/PAD4. Furthermore, knocking out Fg28 had no effect on morphology or pathogenicity. In conclusion, we have identified a set of F. graminearum secreted proteins in the wheat apoplast and a metalloproteinase that triggers immune response, providing new insights into understanding the interaction between F. graminearum and wheat.

Details

Title: Proteomic Analysis of the Fusarium graminearum Secretory Proteins in Wheat Apoplast Reveals a Cell-Death-Inducing M43 Peptidase
Authors/Creators: Pengfeng Li - Agricultural Genomics Institute at Shenzhen
Ruihua Zhao - Agricultural Genomics Institute at Shenzhen
Ying Fang - Huazhong Agricultural University
Yujin Fan - Agricultural Genomics Institute at Shenzhen
Qianyong Hu - Agricultural Genomics Institute at Shenzhen
Wei Huang - South China Agricultural University
Wujun Ma - Murdoch University, Centre for Crop and Food Innovation
Cuijun Zhang - Agricultural Genomics Institute at Shenzhen
Publication Details: Journal of fungi (Basel), Vol.11(4), 240
Publisher: MDPI
Number of pages: 17
Grant note: 110243160001008 / Elite Young Scientists Program of the CAAS AGIS-ZDKY202002 / Shenzhen Science and Technology Program
Identifiers: 991005774733707891
Murdoch Affiliation: Centre for Crop and Food Innovation; School of Agricultural Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.419 Jasmonic Acid
Web Of Science research areas: Microbiology; Mycology
ESI research areas: Plant & Animal Science