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Journal article
Integrative multi-omics data provide insights into the biosynthesis of furanocoumarins and mechanisms regulating their accumulation in Angelica dahurica
Communications biology, Vol.8(1), 649
2025
PMID: 40269101
Abstract
Furocoumarins (FCs), important natural compounds with biodefense roles and pharmacological activities, are notably abundant in medicinal plant Angelica dahurica. However, its accumulation patterns over development stages in FC-enriched tissue, biosynthetic pathways, and regulatory mechanisms in A. dahurica remain elusive. Here, we quantified the concentration dynamics of 17 coumarins across six developmental stages of root and found a gradual decrease in FC concentration as the roots develop. Using a de-novo assembled chromosome-level genome for A. dahurica, we conducted integrative multi-omics analyses to screen out candidate genes to fill in the sole missing step in the biosynthesis of imperatorin and isoimperatorin. This revealed that CYP71AZ18 catalyzes hydroxylation at the C-5 position of psoralen to generate bergaptol, while CYP71AZ19 and CYP83F95 catalyze hydroxylation at the C-8 position to produce xanthotoxol, notably indicating that a single step is catalyzed by two genes from distinct CYP450 subfamilies in this species. CYP71AZ19 originated from a proximal duplication event of CYP71AZ18, specific to A. dahurica, and subsequently underwent neofunctionalization. Accessible chromatin regions (ACRs), especially proximal ACRs, correlated with high gene expression levels, and the three validated genes exhibited strong signals of ACRs, showing the importance of chromosomal accessibility in regulating metabolite biosynthesis.
Details
- Title
- Integrative multi-omics data provide insights into the biosynthesis of furanocoumarins and mechanisms regulating their accumulation in Angelica dahurica
- Authors/Creators
- Jiaojiao Ji - Agricultural Genomics Institute at ShenzhenXiaoxu Han - Agricultural Genomics Institute at ShenzhenLanlan Zang - Agricultural Genomics Institute at ShenzhenYushan Li - Institute of Crop SciencesLiqun Lin - Agricultural Genomics Institute at ShenzhenDonghua Hu - Agricultural Genomics Institute at ShenzhenShichao Sun - Agricultural Genomics Institute at ShenzhenYonglin Ren - Murdoch University, Centre for Biosecurity and One HealthGarth Maker - Murdoch University, Centre for Computational and Systems MedicineZefu Lu - Institute of Crop SciencesLi Wang - Agricultural Genomics Institute at Shenzhen
- Publication Details
- Communications biology, Vol.8(1), 649
- Publisher
- Springer Nature; BERLIN
- Number of pages
- 15
- Grant note
- 32300223, 32070242, and 82373837 / National Natural Science Foundation of China (National Science Foundation of China)
- Identifiers
- 991005765128807891
- Copyright
- © The Author(s) 2025
- Murdoch Affiliation
- Centre for Biosecurity and One Health; School of Medical, Molecular and Forensic Sciences; College of Environmental and Life Sciences; Centre for Computational and Systems Medicine
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.16 Phytochemicals
- 3.16.1717 Bioactive Coumarins
- Web Of Science research areas
- Biology
- ESI research areas
- Biology & Biochemistry