Journal article
Genome-wide identification and validation of target genes associated with insecticide treatment of the green peach aphid, Myzus persicae
Methods in Molecular Biology, Vol.2360, pp.119-138
2022
Abstract
Next-generation sequencing and analyses of whole-genome transcripts can be used to identify genes and potential mechanisms that may be responsible for the development of resistance to insecticides. Such genes can be identified by isolating and sequencing high-quality messenger RNA and identifying differentially expressed genes (DEGs), and gene variants from insecticide-treated and untreated colonies of the Green peach aphid (GPA) or resistant and susceptible GPA populations. Datasets generated would reveal a set of genes whose expression may be associated with the insecticide treatment. The DEGs can then be validated using quantitative PCR assays.
Details
- Title
- Genome-wide identification and validation of target genes associated with insecticide treatment of the green peach aphid, Myzus persicae
- Authors/Creators
- S. Iqbal (Author/Creator) - Murdoch UniversityJ. Fosu-Nyarko (Author/Creator) - Murdoch UniversityF. Brigg (Author/Creator) - Murdoch UniversityM.G.K. Jones (Author/Creator) - Murdoch University
- Publication Details
- Methods in Molecular Biology, Vol.2360, pp.119-138
- Publisher
- Springer Nature
- Identifiers
- 991005541928707891
- Copyright
- © 2022. The Author(s), under exclusive license to Springer Science+Business Media
- Murdoch Affiliation
- College of Science, Health, Engineering and Education; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
Metrics
115 Record Views