Journal article
Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus
Veterinary Immunology and Immunopathology, Vol.149(3-4), pp.167-176
2012
Abstract
Jembrana disease virus (JDV) is an unusual bovine lentivirus that causes an acute and sometimes fatal disease after a short incubation period in Bali cattle (Bos javanicus). The pathological changes occur primarily in lymphoid tissues, which feature proliferating lymphoblastoid-like cells predominantly throughout parafollicular (T-cell) areas, and atrophy of follicles (B-cell) areas. Five Bali cattle were experimentally infected with JDV and all developed typical clinical signs of Jembrana disease characterised by a transient febrile response, enlargement of superficial lymph nodes and a significant leukopenia. Flow cytometric analysis of PBMC during the acute (febrile) disease phase showed that the reduced number of lymphocytes was due to a significant decrease in both the proportion and absolute numbers of CD4 + T cells, but not CD8 + T-cells or CD21 + B-cells. At the end of the febrile phase, total numbers of both CD8 + T-cells and CD21 + B-cells increased significantly, while CD4 + T-cell numbers remained below normal values, resulting in a significantly reduced CD4 +:CD8 + ratio. We speculate that the persistent depletion of CD4 + T cells following JDV infection, through lack of CD4 + T cell help to B cells, may explain the lack of production of JDV-specific antibodies for several weeks after recovery despite an increase in CD21 + B cell numbers. Further, our previous data showing that IgG + plasma cells are targets for JDV infection, correlated with our current data demonstrating an increase in CD8 + T cell numbers, supports the suggestion that anti-viral cytotoxic T cell or other cell-mediated immune responses may be critical in the recovery process, although this remains to be formally demonstrated for JDV.
Details
- Title
- Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus
- Authors/Creators
- I.W. Tenaya (Author/Creator) - Murdoch UniversityK. Heel (Author/Creator) - School of Pathology and Laboratory MedicineP.A. Stumbles (Author/Creator) - Murdoch UniversityG.E. Wilcox (Author/Creator) - Murdoch University
- Publication Details
- Veterinary Immunology and Immunopathology, Vol.149(3-4), pp.167-176
- Publisher
- Elsevier BV
- Identifiers
- 991005541154107891
- Copyright
- © 2012 Elsevier B.V.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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