Conference presentation
MALDI-TOF approach to measure cysteine number of wheat glutenin subunits
11th International Gluten Workshop (Fragrant Hill Empark Hotel, Beijing, China, 12/08/2012–16/08/2012)
2012
Abstract
In the current study, we established a fast method to accurately measure the number of cysteine residues in high molecular weight glutenin subunits. An alkylation regent, 4-vinylpyridine (4-vp), was used to treat the proteins during extraction. For every cysteine residue in a protein, such treatment increases its molecular mass value by 105.14 Da, which can be accurately determined by MALDI-TOF equipment. Based on the changes of the molecular mass value after 4-vp treatment, the number of cysteine residue can be reliably determined. We found that this method is also useful in studying non-glutenin proteins such as lupin seed storage proteins. This method is particularly valuable when the number of cysteine residue is of importance.
Details
- Title
- MALDI-TOF approach to measure cysteine number of wheat glutenin subunits
- Authors/Creators
- W. Ma (Author/Creator)K. Wang (Author/Creator)J. Ma (Author/Creator)S. Islam (Author/Creator)R. Appels (Author/Creator)F. Bekes (Author/Creator)Y. Yan (Author/Creator)
- Conference
- 11th International Gluten Workshop (Fragrant Hill Empark Hotel, Beijing, China, 12/08/2012–16/08/2012)
- Identifiers
- 991005543769307891
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Conference presentation
Metrics
98 Record Views